Lytic enzymes toward aniline-assimilating Rhodococcus erythropolis AN-13: Purification and characterization.
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Expression of bovine lactoferrin C-lobe in Rhodococcus erythropolis and its purification and characterization.
A Rhodococcus erythropolis expression system for the bovine lactoferrin C-lobe was constructed. The DNA fragments encoding the BLF C-lobe were amplified and cloned into vector pTip LCH1.2. R. erythropolis carrying the pTip-C-lobe was cultured at 30 degrees C with shaking, and expression of the rBLF C-lobe was induced by adding 1 microg/ml (final concentration) thiostrepton. The rBLF C-lobe was ...
متن کاملPurification and Characterization f Two Muconate Cycloisomerase from Aniline - assimilating 1 lyateuria Species ANA - 18
Two muconate cycloisomerases (MC I and MC II, EC 5.5.1.1> were purified to homogeneity from a" anilinegrown R'ateuria sp. ANA-18. MC I and MC II were similar in molecular mass, optimal pH, and pH stability but differny ent in thermostability, and some other enzymatic properties. NH2-terminal amino acid sequences were different between the twe isozymes, indicated that these are encoded by differ...
متن کاملIsolation and Characterization of Carbendazim-degrading Rhodococcus erythropolis djl-11
Carbendazim (methyl 1H-benzimidazol-2-yl carbamate) is one of the most widely used fungicides in agriculture worldwide, but has been reported to have adverse effects on animal health and ecosystem function. A highly efficient carbendazim-degrading bacterium (strain dj1-11) was isolated from carbendazim-contaminated soil samples via enrichment culture. Strain dj1-11 was identified as Rhodococcus...
متن کاملCharacterization of catechol catabolic genes from Rhodococcus erythropolis 1CP.
The biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing Rhodococcus erythropolis strain 1CP previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. Based on sequences of the N terminus and of tryptic peptides of the muconate ...
متن کاملCharacterization of LtsA from Rhodococcus erythropolis, an enzyme with glutamine amidotransferase activity.
The nocardioform actinomycete Rhodococcus erythropolis has a characteristic cell wall structure. The cell wall is composed of arabinogalactan and mycolic acid and is highly resistant to the cell wall-lytic activity of lysozyme (muramidase). In order to improve the isolation of recombinant proteins from R. erythropolis host cells (N. Nakashima and T. Tamura, Biotechnol. Bioeng. 86:136-148, 2004)...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.52.935